FIELD: purification of botulinum toxin.
SUBSTANCE: invention relates to a method for purifying botulinum toxin, comprising (a) acid precipitation or ultrafiltration of a culture solution containing botulinum toxin type A; (b) purifying the pre-treated botulinum toxin by cation exchange chromatography using an SP column; (c) purification of botulinum toxin by hydrophobic chromatography using a phenyl column; and (d) purifying the botulinum toxin by anion exchange chromatography using a Q column.
EFFECT: method allows the botulinum toxin to be purified to a high degree of purity.
11 cl, 7 dwg, 9 tbl, 6 ex
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