METHOD FOR IMPROVING SAFETY AND EFFICIENCY OF STORAGE AND TRANSPORTATION OF A TRANSPLANTED ORGAN UNDER PRESSURE OF A PRESERVING GAS MIXTURE AND A DEVICE BASED THEREON Russian patent published in 2019 - IPC A01N1/02 

FIELD: biotechnology.

SUBSTANCE: invention refers to biotechnology, namely to safe storage and transportation of transplanted cooled heart animals under pressure of preserving gaseous medium and to a mobile device for this purpose. Method involves preparation of a transplant for storage by perfusion of a transplanted organ using a storage solution in the group: Tyrode solution with addition of ceftriaxone 0.1 mg/ml, Euro-Collins solution with addition of dimethyl sulphoxide 0.5 vol%, dexamethasone 16 mcg/ml and cefalosin 0.1 mg/ml, followed by placing the graft in a container in a transfusion perfusion, storage and transportation device body, wherein air is substituted in container with graft preserving gaseous medium, consisting of mixture of CO and O₂ gases in ratio of 4:3, pre-arranged in chamber with variable volume, where gas pressure of 7 atm per graft is provided by displacement of preserving gaseous medium from inner chamber volume, which is in form of chamber with elastic shell or in form of cylinder with movable piston, by forming pressure on outer part of said chamber by means of external compressor using ambient air. Mobile device comprises a body with a container, in which a transplanted organ, a thermostat, pressure and temperature sensors, a perfusion system comprising N reservoirs containing solutions for organ perfusion or storage, wherein the reservoirs inputs through the air valves and the air splitter are connected to the mini-compressor output with the possibility to displace the selected solution from the reservoir volume, and the reservoirs outlets are connected to the liquid splitter inlets the output of which is connected to the spring valve with the possibility to supply the selected solution to the transplanted organ, wherein the device further comprises a variable-volume chamber configured to pre-arrange therein a preserving gaseous medium, the air outlet of which through the air valve is connected to the air inlet of the graft container, where the pressure of gases on the graft is provided by forming an external pressure on the outer shell of the chamber with a variable volume, device further comprises a bogie on which a thermostat is mounted, in which a housing with a container, a control unit, a battery, an external compressor is installed, the output of which is connected to the inner volume of the housing with the possibility of creating pressure inside the housing, where the control unit input receives signals from a temperature sensor and a pressure sensor arranged inside the housing, wherein the control unit controls the crane switching, the mini compressor operation, processes signals from the sensors according to the program placed in the storage unit of the unit, with the possibility of supplying emergency signals.

EFFECT: invention allows to increase safety and efficiency of donor organ long storage and transportation.

18 cl, 2 dwg, 7 ex