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2 710 065

(13)

(51)

IPC

C12Q1/00(2006-01-01)

C12Q1/68(2006-01-01)

(21) (22)

Application

2018144588, 2018-12-14

(24)

Start date

2018-12-14

(22)

Actual filing date

2018-12-14

(45)

Published

2019-12-24

(72)

Inventor

Elsukova Aleksandra AndreevnaVlasova Natalya NikiforovnaIgolkin Aleksej Sergeevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING DNA OF ASF VIRUS BY LOOP ISOTHERMAL AMPLIFICATION Russian patent published in 2019 - IPC C12Q1/00 C12Q1/68

Abstract RU 2710065 C1

FIELD: veterinary medicine.

SUBSTANCE: invention relates to veterinary virology, specifically to molecular diagnostics. There are developed oligonucleotide primers for detecting DNA of African swine fever virus: F3 p22 GTTTTACGGATACTGCTGC, B3 p22 CCTTACCATATTTAAGAACCGG, FIP p22 TGGGGGCTATGGGATTCACT-AAGAATTTGGAAAAACACGGC, BIP p22 TGTGTGAAAAATATTGTTCATGGGG-ACATAACATGTTCCCTCCTT, LoopB p22 CCGATGACTGTACAGGTTGGG. Disclosed also is a method for detecting DNA of African swine fever virus from biological material using modified PCR, specifically loop isothermal amplification (LAMP).

EFFECT: present invention enables fast and high-reliability detection of the presence of DNA of ASF virus by loop isothermal amplification when examining samples of a wide range of pathological and biological material in field conditions and laboratories.

2 cl, 4 tbl, 2 ex

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RU 2 710 065 C1

Authors

Elsukova Aleksandra Andreevna

Vlasova Natalya Nikiforovna

Igolkin Aleksej Sergeevich

Dates

2019-12-24—Published

2018-12-14—Filed