FIELD: veterinary medicine.
SUBSTANCE: invention relates to veterinary virology, specifically to molecular diagnostics. There are developed oligonucleotide primers for detecting DNA of African swine fever virus: F3 p22 GTTTTACGGATACTGCTGC, B3 p22 CCTTACCATATTTAAGAACCGG, FIP p22 TGGGGGCTATGGGATTCACT-AAGAATTTGGAAAAACACGGC, BIP p22 TGTGTGAAAAATATTGTTCATGGGG-ACATAACATGTTCCCTCCTT, LoopB p22 CCGATGACTGTACAGGTTGGG. Disclosed also is a method for detecting DNA of African swine fever virus from biological material using modified PCR, specifically loop isothermal amplification (LAMP).
EFFECT: present invention enables fast and high-reliability detection of the presence of DNA of ASF virus by loop isothermal amplification when examining samples of a wide range of pathological and biological material in field conditions and laboratories.
2 cl, 4 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND A METHOD OF HIGHLY SENSITIVE DETECTION OF AFRICAN SWINE FEVER VIRUS DNA BY LOOP ISOTHERMAL AMPLIFICATION IN THE PRESENCE OF INTERNAL CONTROL SAMPLE DNA | 2022 |
|
RU2799410C1 |
| METHOD FOR DETECTING AFRICAN SWINE FEVER VIRUS DNA USING SPECIFIC OLIGONUCLEOTIDES BY ISOTHERMAL LOOP AMPLIFICATION AND COLORIMETRIC DETECTION OF AMPLIFICATION RESULTS | 2022 |
|
RU2806908C1 |
| OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT PROBE WITH INNER DAMPER, COMPLEMENTARY TO THE GENE SECTION P30 (CP204L) OF THE AFRICAN SWINE FEVER VIRUS, FOR USE IN POLYMERASE CHAIN REACTION IN REAL TIME | 2016 |
|
RU2606253C1 |
| SET OF HIGHLY SPECIFIC OLIGONUCLEOTIDE PRIMERS AND PROBES FOR DETECTION AND DIFFERENTIATION OF ASF, CSF AND VD VIRUSES | 2024 |
|
RU2828887C1 |
| METHOD FOR EDITING DNA OF AFRICAN SWINE FEVER VIRUS IN AREA OF GENES MGF 110_4L-6L USING PLASMIDS U6, pJET1.2 AND ENZYME Cas9 | 2023 |
|
RU2828403C1 |
| KIT OF OLIGONUCLEOTIDE PRIMERS FOR GENERATION OF LIBRARY OF FULL-LENGTH GENES CODING IMMUNODOMINANT PROTEINS P17 (D11L), P30 (CP204L), P54 (E183L), P60 (CP530R), P72 (B646L) OF AFRICAN SWINE FEVER VIRUS | 2014 |
|
RU2571855C2 |
| RECOMBINATIONAL CARTRIDGE CONTAINING THE EP153R AND EP364R GENES OF THE CONGO (KK-262) STRAIN OF THE AFRICAN SWINE FEVER VIRUS AND RECOMBINANT ΔCONGOCD2V STRAIN OF THE AFRICAN SWINE FEVER VIRUS | 2016 |
|
RU2654586C2 |
| RECOMBINATIONAL CARTRIDGE CONTAINING GENES EP153R AND EP402R OF STRAIN F-32 OF AFRICAN SWINE FEVER VIRUS AND RECOMBINANT STRAIN DSWCONGO/FRANCELECTINCD2 OF AFRICAN SWINE FEVER VIRUS | 2014 |
|
RU2571858C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING RNA OF YELLOW FEVER VIRUS BY ISOTHERMAL AMPLIFICATION IN REAL TIME | 2024 |
|
RU2824209C1 |
| STRAIN "ASF/PRIMORSKY/DP-4560_REC/2023" OF RECOMBINANT AFRICAN SWINE FEVER VIRUS FOR DEVELOPMENT AND MANUFACTURE OF DIAGNOSTIC TOOLS | 2024 |
|
RU2835908C1 |
