FIELD: biotechnology.
SUBSTANCE: invention relates to a method for editing DNA of the African swine fever virus in MGF 110_4L-6L genes using plasmids U6, pJET1.2 and Cas9 enzyme. Said method provides for the creation of a plasmid-guide (gRNA) based on the plasmid U6, containing 20 bps, complementary to the portion of the genome of the ASF virus with genes MGF 110_4L-6L in positions 8724-10107 nucleotide base of the genome of the ASF virus, creation of shuttle plasmid Shuttle plasmid based on plasmid pJET1.2, which contains two fragments of ASF virus genome—Left-arm (L-arm) and Right-arm (R-arm), flanking MGF 110_4L-6L genes for their deletion, ASF virus genome editing in MGF 110_4L-6L genes region and the selected ASF virus strain in the primary porcine spleen cell culture, extraction of ASF virus DNA, sequencing and confirmation of MGF 110_4L-6L gene deletion.
EFFECT: invention is effective for editing DNA of African swine fever virus in the area of MGF 110_4L-6L genes using plasmids U6, pJET 1.2 and Cas9 enzyme.
1 cl, 5 dwg, 4 tbl, 4 ex
| Title |
Year |
Author |
Number |
| RECOMBINATIONAL CARTRIDGE CONTAINING GENES EP153R AND EP402R OF STRAIN F-32 OF AFRICAN SWINE FEVER VIRUS AND RECOMBINANT STRAIN DSWCONGO/FRANCELECTINCD2 OF AFRICAN SWINE FEVER VIRUS |
2014 |
- Burmakina Galina Sergeevna
- Morgunov Jurij Petrovich
- Titov Il'Ja Andreevich
- Mima Ksenija Aleksandrovna
- Kolbasov Denis Vladimirovich
- Malogolovkin Aleksandr Sergeevich
|
RU2571858C1 |
| RECOMBINATIONAL CARTRIDGE CONTAINING THE EP153R AND EP364R GENES OF THE CONGO (KK-262) STRAIN OF THE AFRICAN SWINE FEVER VIRUS AND RECOMBINANT ΔCONGOCD2V STRAIN OF THE AFRICAN SWINE FEVER VIRUS |
2016 |
- Burmakina Galina Sergeevna
- Morgunov Yurij Petrovich
- Titov Ilya Andreevich
- Mima Kseniya Aleksandrovna
- Kolbasov Denis Vladimirovich
- Malogolovkin Aleksandr Sergeevich
|
RU2654586C2 |
| ACHS/VNIIZZH/CV-1 STRAIN OF AFRICAN SWINE FEVER, WITH DECREASED VIRULENCE FOR PIGS, FOR VIROLOGIC, DIAGNOSTIC, MOLECULAR-GENETIC AND SURVEILLANCE STUDIES |
2018 |
- Vlasova Natalya Nikiforovna
- Zhukov Ivan Yurevich
- Mazlum Ali
- Sharypova Darya Viktorovna
- Pershin Andrej Sergeevich
- Igolkin Aleksej Sergeevich
|
RU2675535C1 |
| ATTENUATED ASFV/CV60/2020 STRAIN OF AFRICAN SWINE FEVER VIRUS OF THE ASFARVIRIDAE FAMILY, GENUS ASFIVIRUS FOR THE STUDY OF IMMUNOLOGICAL REACTIONS, MOLECULAR GENETIC ANALYSIS, GENETIC MODIFICATION AND CREATION OF A PROTOTYPE VACCINE AGAINST ASF |
2021 |
- Vlasova Natalya Nikiforovna
- Igolkin Aleksej Sergeevich
- Mazlum Ali
- Pershin Andrej Sergeevich
- Zhukov Ivan Yurevich
- Shevchenko Ivan Vyacheslavovich
- Shotin Andrej Romanovich
- Elsukova Aleksandra Andreevna
- Aronova Elena Vladimirovna
|
RU2760399C1 |
| KIT OF OLIGONUCLEOTIDE PRIMERS FOR GENERATION OF LIBRARY OF FULL-LENGTH GENES CODING IMMUNODOMINANT PROTEINS P17 (D11L), P30 (CP204L), P54 (E183L), P60 (CP530R), P72 (B646L) OF AFRICAN SWINE FEVER VIRUS |
2014 |
- Mima Ksenija Aleksandrovna
- Burmakina Galina Sergeevna
- Titov Il'Ja Andreevich
- Malogolovkin Aleksandr Sergeevich
|
RU2571855C2 |
| OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT PROBE WITH INNER DAMPER, COMPLEMENTARY TO THE GENE SECTION P30 (CP204L) OF THE AFRICAN SWINE FEVER VIRUS, FOR USE IN POLYMERASE CHAIN REACTION IN REAL TIME |
2016 |
- Kudryashov Dmitrij Andreevich
- Burmakina Galina Sergeevna
- Titov Ilya Andreevich
- Tsybanov Sodnom Zhamyanovich
- Malogolovkin Aleksandr Sergeevich
|
RU2606253C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND A METHOD OF HIGHLY SENSITIVE DETECTION OF AFRICAN SWINE FEVER VIRUS DNA BY LOOP ISOTHERMAL AMPLIFICATION IN THE PRESENCE OF INTERNAL CONTROL SAMPLE DNA |
2022 |
- Gnezdilova Larisa Aleksandrovna
- Borunova Saidfatima Mirovna
- Davydova Ekaterina Evgenevna
- Selina Marina Viktorovna
|
RU2799410C1 |
| METHOD OF PRODUCTION OF MONOCLONAL ANTIBODIES TO PROTEIN P30 OF AFRICAN SWINE FEVER VIRUS USING RECOMBINANT CONSTRUCTIONS |
2013 |
- Pershin Andrej Sergeevich
- Kazakova Anna Sergeevna
- Kapustina Ol'Ga Vladimirovna
- Vlasova Natal'Ja Nikiforovna
|
RU2534343C1 |
| METHOD OF INFECTIOUS BOVINE LUMPY SKIN DISEASE VIRUS GENOME EDITING USING OVERLAP-PCR AND CRISPR-CAS9 TECHNOLOGY |
2023 |
- Sprygin Aleksandr Vladimirovich
- Mazlum Ali
- Biadovskaia Olga Petrovna
- Doronin Maksim Igorevich
- Chvala Ilia Aleksandrovich
- Shumilova Irina Nikolaevna
|
RU2825451C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING DNA OF ASF VIRUS BY LOOP ISOTHERMAL AMPLIFICATION |
2018 |
- Elsukova Aleksandra Andreevna
- Vlasova Natalya Nikiforovna
- Igolkin Aleksej Sergeevich
|
RU2710065C1 |
