FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, namely, to synthetic oligonucleotide probes and primers for detection of DNA of african swine fever virus. Offered synthetic oligonucleotide primers and fluorescence marked probe with inner damper complementary to the conservative region of the gene P30 (CP204L) of the african swine fever virus and have the following nucleotide composition: F1 p30 5'-GTTACGACCGCTATAAAAACA-3'; R1 p30 5'-TTCCATTCTTCTTGAGACCTG-3'; Z1 (int) p30 5'-(FAM)TACTGTT(RTQ1)AAGTATGATATTGTGA(BHQ1)-3'.
EFFECT: proposed invention can be used in gene diagnostics of infectious diseases of pigs and veterinary virology.
1 cl, 4 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| KIT OF OLIGONUCLEOTIDE PRIMERS FOR GENERATION OF LIBRARY OF FULL-LENGTH GENES CODING IMMUNODOMINANT PROTEINS P17 (D11L), P30 (CP204L), P54 (E183L), P60 (CP530R), P72 (B646L) OF AFRICAN SWINE FEVER VIRUS | 2014 |
|
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