METHODS OF PERFUSION CULTIVATION AND USE THEREOF Russian patent published in 2020 - IPC C12N5/00 

Abstract RU 2733539 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention provides a method for testing the presence of a contaminant in a first or second liquid culture medium, an initial material used to produce a first or second liquid culture medium, or a mammalian cell source containing a nucleic acid which encodes a recombinant protein, wherein the method involves: providing a multi-well plate, incubating a multi-well plate, detecting recombinant protein in a mammalian cell or in a first and/or second liquid culture medium; comparing the amount of recombinant protein, identifying a first or second liquid culture medium, a raw material used to produce a first or second liquid culture medium, or a mammalian cell source, as containing a contaminant, when the level of the produced recombinant protein is below the reference level.

EFFECT: invention can be used for high-efficiency screening of tissue culture media and components of tissue culture media.

17 cl, 2 ex, 10 tbl, 29 dwg

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RU 2 733 539 C2

Authors

Villiger-Oberbek, Agata

Yang, Jianguo

Yang, Yang

Lornzo, Gabrielle

Dates

2020-10-05Published

2015-06-05Filed