FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Described is a method of producing murine monoclonal antibodies specific to different strains of influenza virus B of Yamagata- and Victoria-lineage and having high specificity to NP-protein, comprising steps of producing antibody-producing splenocytes; murine myeloma cell accumulation; fusion of antibody-producing splenocytes with myeloma cells; selection of hybridomas; inoculating the hybridoma cells in the abdominal cavity of primed mice; accumulation of monoclonal antibodies in ascites; purification of obtained monoclonal antibodies. In the above method, for producing antibodies, a hybridoma-producing monoclonal antibody directed to NP-protein of influenza B virus, deposited as 6G1/B under RKKK(P) 785D (Russian collection of vertebrate cell cultures), or a hybridoma-producing monoclonal antibody directed to NP-protein of influenza B virus, deposited as 10A5/V under number RKKK(P) 787D in Specialized collection of cell cultures of the Collection of Vertebrate Cell Cultures Core facilities centre of the Federal Publicly Funded Institution of Science of the Institute of Cytology of the Russian Academy of Sciences.
EFFECT: invention extends the range of methods for producing murine monoclonal antibodies specific for influenza virus B of Yamagata- and Victoria-lineage and having high specificity for NP-protein.
3 cl, 8 dwg, 1 tbl, 5 ex
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