METHOD OF ASSESSING BACTERIOPHAGE SPECIFIC ACTIVITY USING CELL CULTURES Russian patent published in 2020 - IPC C12Q1/70 C12Q1/02 C12Q1/18 

Abstract RU 2723188 C1

FIELD: medicine.

SUBSTANCE: invention refers to medicine, microbiology, biology. Disclosed is a method of evaluating specific activity of bacteriophages in standard conditions of a monolayer of cell culture of pulmonary fibroblasts of human embryo (PHE-3) with evaluation of results by degree of reduction of adhesion of test strain of microorganism to cells of monolayer and degree of lysis of non-adherent bacterial cells, providing for 1 hour of combined cultivation of test strain of microorganism with corresponding bacteriophage in amount of 0.2 ml. That is followed by evaluating the result compared to the bacteriophage-free control.

EFFECT: invention provides reduced reaction time.

1 cl, 4 tbl, 1 ex

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RU 2 723 188 C1

Authors

Alimov Aleksandr Viktorovich

Zakharova Yuliya Aleksandrovna

Fedotova Olga Semenovna

Shmeleva Natalya Anatolevna

Dates

2020-06-09Published

2019-04-27Filed