DEOXYURIDINE TRIPHOSPHATES MARKED WITH ZWITTERIONIC INDOCYANINE DYES Russian patent published in 2020 - IPC C07H19/73 C09B23/06 C12Q1/68 G01N33/58 

FIELD: chemistry.

SUBSTANCE: present invention refers to used as fluorescent labels for enzymatic marking DNA during PCR nucleoside triphosphate and intermediate compounds for their production. Disclosed are compounds of formulas:

, ,

where Y is -(CH₂)₃-; -(CH₂)₅; -(CH₂)₅CONH(CH₂)₃-; X is -CH₂-; -CH₂CONH(CH₂)₅-; -CH₂CONH(CH₂)₅CONH(CH₂)₅-; M⁺ represents H⁺, Na⁺, Li⁺, K⁺, [CH₃(CH₂)_n]_(4-m)NH_m⁺, where n=0–5, m=0–4. Disclosed are compounds of formulas:

, , ,

where R is -OH; -NH(CH₂)₅COOH; -NH(CH₂)₅CONH(CH₂)₅COOH; m=3–5. Disclosed is a compound of formula

,

where R is -NH(CH₂)₅COOH; -NH(CH₂)₅CONH(CH₂)₅COOH at m=3 or R is -OH; -NH(CH₂)₅COOH; -NH(CH₂)₅CONH(CH₂)₅COOH at m=5.

EFFECT: disclosed are novel compounds which are effective as fluorescent labels for enzymatic marking of DNA during PCR, as well as novel compounds for preparing said compounds.

13 cl, 43 ex, 3 tbl, 5 dwg