FIELD: medicine.
SUBSTANCE: invention relates to molecular biology, microbiology and medicine. Invention includes a set of original oligonucleotide primers for method implementation, as well as an algorithm for selecting primers which are non-specific to the detected DNA fragments and capable of amplifying another fragment of the blaTEM gene in the analysed sample.
EFFECT: invention provides a method for eliminating false-positive results caused by contamination of DNA polymerases when detecting blaTEM genes of resistance to β-lactam antibiotics.
2 cl, 2 dwg, 1 tbl, 1 ex
