FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a method of extracting natural antimicrobial peptides (AMP) from leukocyte-erythrocyte-platelet blood mass. Used is a leukocyte-erythrocyte-platelet blood mass pre-exposed to trypsin haemolysis. Separation of AMP is carried out by liquid chromatography on a separating column with three-fold use of Sephadex G-25.
EFFECT: invention enables to optimize the technology of extracting the most complete fraction of natural low-molecular weight peptides containing AMP, to use Sephadex G-25 several times after washing, regeneration and drying, to increase their biological value and to reduce the cost of production thereof.
1 cl, 2 tbl, 3 dwg
Title | Year | Author | Number |
---|---|---|---|
METHOD OF ISOLATING ENDOGENOUS ANTIMICROBIAL PEPTIDES FROM PLACENTAL TISSUE | 2022 |
|
RU2813979C2 |
OPTIMIZED RELEASE OF ENDOGENOUS BETA-DEFENSINS | 2024 |
|
RU2825572C1 |
NIOSOMAL ANTIMICROBIAL GEL FOR TREATING DIABETIC ULCERS, WOUNDS, BURNS, INCLUDING THOSE INFECTED WITH ANTIBIOTIC-RESISTANT MICROORGANISMS | 2021 |
|
RU2781402C2 |
METHOD FOR FRACTIONATION OF LEUKOCYTE PROTEINS | 2019 |
|
RU2737730C2 |
SEPARATION METHOD OF LOW-MOLECULAR PEPTIDES | 2012 |
|
RU2510398C2 |
METHOD OF PRODUCING BIOLOGICALLY ACTIVE PEPTIDE-AMINO ACID PREPARATION BASED ON HUMAN BLOOD PLASMA | 2022 |
|
RU2799637C1 |
METHOD OF EXTRACTING LOW MOLECULAR PEPTIDES | 2008 |
|
RU2416243C2 |
ANTIBACTERIAL AGENT AND PHARMACEUTICAL COMPOSITION COMPRISING EFFECTIVE AMOUNT OF ANTIBACTERIAL AGENT | 2005 |
|
RU2278675C1 |
METHOD FOR ANTIMICROBIAL PEPTIDE CECROPIN P1 PRODUCTION FROM PINNATED KALANCHOE TRANSGENIC PLANTS EXTRACT | 2016 |
|
RU2632116C1 |
BACILLUS SUBTILIS STRAIN - PRODUCER OF A LOW-MOLECULAR ANTIMICROBIAL PEPTIDE AND METHOD FOR PRODUCING A LOW-MOLECULAR ANTIMICROBIAL PEPTIDE | 2020 |
|
RU2758060C1 |
Authors
Dates
2020-08-04—Published
2019-10-29—Filed