FIELD: biochemistry.
SUBSTANCE: invention relates to a method of producing secondary stem cell clones from megakaryocyte precursor cells, providing dedifferentiation of precursors of megakaryocytes for formation of a colony of stem cells, as well as separation of secondary stem cells from it. Also disclosed is a method of producing megakaryocyte precursor cells, providing induction of differentiation of recovered clones of secondary stem cells into precursors of megakaryocytes. Invention also relates to a method for producing thrombocytes, providing maturing of the megakaryocyte precursor cells maturation in the megakaryocytes and thrombocyte release.
EFFECT: invention enables efficient production of thrombocytes from megakaryocyte progenitor cells.
10 cl, 11 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NOVEL METHOD FOR PRODUCING DIFFERENTIATED CELLS | 2010 |
|
RU2661107C1 |
| NOVEL METHOD FOR PRODUCING DIFFERENTIATED CELLS | 2010 |
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| METHOD FOR OBTAINING CULTURE CONTAINING MEGAKARYOCYTES AND METHOD FOR OBTAINING PLATELETS WITH ITS APPLICATION | 2016 |
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| METHOD FOR PRODUCING HIGH-FUNCTION THROMBOCYTES | 2016 |
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| METHOD FOR THE PRODUCTION OF PLATELETS BY A METHOD FOR ROTATIONAL MIXING OF THE CULTURE | 2016 |
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| METHOD OF PRODUCING THROMBOCYTES BY MEANS OF DEVICE FOR BACK-AND-FORTH STIRRING | 2016 |
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RU2741871C2 |
| METHOD FOR PRODUCING POLYPLOIDIZED MEGAKARYOCYTE AND PLATELETS | 2012 |
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RU2606764C2 |
| METHOD OF PRODUCTION OF INDUCED PLURIPOTENT STEM CELLS OF PATIENTS WITH DOWN SYNDROME | 2012 |
|
RU2492233C1 |
| METHOD FOR PRODUCING PURIFIED THROMBOCYTES | 2016 |
|
RU2766177C2 |
| CONVERSION OF SOMATIC CELLS INTO INDUCED REPROGRAMMED NEURAL STEM CELLS (IRNSC) | 2011 |
|
RU2562111C2 |
