METHOD FOR PRODUCING PURIFIED THROMBOCYTES Russian patent published in 2022 - IPC A61K35/19 A61K35/14 A61P7/00 

FIELD: hematology.

SUBSTANCE: group of inventions relates to hematology, namely to a method for producing purified thrombocytes from megakaryocyte culture and a method for producing a blood preparation from obtained thrombocytes. The method for producing purified thrombocytes from the megakaryocyte culture includes: removal of megakaryocytes from the megakaryocyte culture by centrifugation of the culture to obtain a liquid component containing thrombocytes separated from megakaryocytes; and purification of thrombocytes in the liquid component by centrifugation of the liquid component, where centrifugation is carried out using a centrifuge separator equipped with: a rotating separation tank proposed with an inner wall, to which substances with a high specific gravity corresponding to the centrifugal force adhere, and with an outlet, through which the liquid component flows out after separation; and production means the extraction of obtained purified thrombocytes that flow out of the outlet, and the addition of a washing solution to the separation tank, and the rotation of the separation tank to remove substances that adhere to and are deposited on the inner wall of the separation tank; and the extraction of purified thrombocytes that adhere to the inner wall of the separation tank by adding a production solution, under certain conditions. A method for producing a blood preparation is also proposed.

EFFECT: above-described method allows to obtain purified high-quality thrombocytes from megakaryocyte culture by separating and purifying a large number of thrombocytes in one loading batch and with a high degree of yield.

4 cl, 5 dwg, 1 tbl, 1 ex