FIELD: veterinary virology.
SUBSTANCE: invention relates to veterinary virology, in particular to molecular diagnostics. A highly sensitive test system has been developed, consisting of an RT-PCR mixture, Taq-DNA-polymerase, reverse transcriptase, positive control and negative control, which makes it possible to quickly detect SARS-CoV-2 virus RNA in biomaterial from animals, food products and environmental objects. Wednesday. Oligonucleotide primers and a fluorescently labeled probe have been developed for amplification and detection of the SARS-CoV-2 virus N gene region:
.
EFFECT: invention can be used to detect the RNA of the SARS-CoV-2 virus in biomaterial from animals, food products and environmental objects in order to establish and clarify the diagnosis in clinical and laboratory diagnostics in veterinary medicine.
2 cl, 1 dwg, 3 tbl, 3 ex
| Title | Year | Author | Number |
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| SET OF OLIGODEOXYRIBONUCLETIDE PRIMERS AND FLUORESCENT-LABELED PROBE FOR IDENTIFICATION OF RNA OF HUMAN CORONAVIRUSES SARS AND 2019-NCOV BY RT-PCR METHOD WITH HYBRIDIZATION-FLUORESCENT DETECTION IN REAL TIME | 2020 |
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|
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| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
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| TEST SYSTEM FOR DETECTING SPECIFIC NUCLEOTIDE SEQUENCES CHARACTERISTIC OF THE DETECTED MICROORGANISMS OR VIRUSES, METHOD FOR APPLICATION OF THE TEST SYSTEM (OPTIONS) | 2021 |
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| SET FOR IDENTIFYING SARS-CoV-2 CORONAVIRUS | 2021 |
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RU2765497C1 |
| TEST SYSTEM FOR DETECTING SARS-CoV-2, INFLUENZA VIRUS A, INFLUENZA VIRUS B BY THE METHOD OF ONE-STEP POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION | 2021 |
|
RU2761481C1 |
| TEST SYSTEM BASED ON REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION FOR THE DETECTION OF SARS-COV-2 LINE OMICRON WITH THE DEFINITION OF SUBVARIANT BA.1 | 2022 |
|
RU2779025C1 |
| TEST SYSTEM AND METHOD FOR DETECTING RNA OF CORONAVIRUS SARS-COV-2, VIRUS-AGENT OF CORONAVIRUS DISEASE 2019 COVID-19, BY POLYMERASE CHAIN REACTION IN REAL TIME (EMBODIMENTS) | 2020 |
|
RU2731390C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFICATION OF HUMAN CORONAVIRUS 2019-nCoV RNA BY PCR WITH HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2020 |
|
RU2734300C1 |
