METHOD FOR DIFFERENTIAL DIAGNOSIS OF CERVICAL INTRAEPITHELIAL NEOPLASIA OF GRADE III AND MICROINVASIVE CERVICAL CANCER ASSOCIATED WITH HUMAN PAPILLOMA VIRUS Russian patent published in 2020 - IPC G01N33/53 A61B5/00 

FIELD: medicine.

SUBSTANCE: invention relates to a method for differential diagnosis of cervical intraepithelial neoplasia III degree and microinvasive cervical cancer (stage IA1 by FIGO) associated with human papilloma virus and can be used in medicine. Disclosed is a method for differential diagnosis of cervical grade III intraepithelial neoplasia and microinvasive cervical cancer (stage IA1 by FIGO) associated with human papilloma virus, involving immunological examination of patient's blood lymphocytes by multiparametric flow cytometry, characterized by that in immunological examination, the number of cells with the phenotype CD3+CD8+PD1+TIM3+ and CD3+CD8+PD1+LAG3+ in the general population of CD3+CD8+ T-lymphocytes is determined, with the number of cells with phenotype CD3+CD8+PD1+TIM3+ and CD3+CD8+PD1+LAG3+ in each of the versions less than 5 % of the total population of CD3+CD8+ T-lymphocytes, cervical grade III intraepithelial neoplasia is diagnosed, with the number of cells with the phenotype CD3+CD8+PD1+TIM3+ and CD3+CD8+PD1+LAG3+ in each of the above cases more than 5 % are diagnosed with microinvasive cancer.

EFFECT: what is presented is a new accurate diagnostic technique for cervical grade III intraepithelial neoplasia and microinvasive cervical cancer (CC) associated with human papilloma virus according to the International Gynecological Classification (FIGO), suitable for implementation of improved approaches to treatment of patients with severe dysplasia and cervical cancer, allowing to maintain a full reproductive function in this category.

1 cl, 2 ex