FIELD: biotechnology.
SUBSTANCE: invention is a method applicable for a different type of biological material, including whole blood and capillary blood samples, a leukocyte film, amniotic fluid, placenta villi, hair bulbs, dry whole blood spots, involving treatment of biological samples with a lysing buffer, wherein incubation is carried out at temperature 98 °C for 15 min, centrifugation—at 13.4 thousand rpm for 75 s, and lysing buffer include 0.75 mM EDTA (pH=8), 35 mcM SDS.
EFFECT: method enables to extract DNA from biological samples with high concentration and high degree of purity of the recovered DNA in a method for rapid isolation of genomic DNA for clinical studies.
1 cl, 4 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR EXTRACTION OF NUCLEIC ACIDS FROM NAIL PLATES | 2020 |
|
RU2751244C1 |
METHOD FOR EXPRESS DNA EXTRACTION FROM UNFROZEN BLOOD | 2018 |
|
RU2679907C1 |
METHOD FOR MYCOPLASMA HOMINIS DNA DETECTION FROM BLOOD SAMPLES | 2013 |
|
RU2533238C1 |
METHOD FOR RNA AND DNA ISOLATION FROM DRY BIOLOGICAL SAMPLES STORED ON PAPER MEDIA AND SET FOR ITS IMPLEMENTATION | 2016 |
|
RU2628695C1 |
MICROBIAL TEST STRAIN OF Helicobacter pylori GKPM-Obolensk V-7215 FOR INDICATION AND IDENTIFICATION OF Helicobacter pylori IN SAMPLES OF BIOPSY MATERIALS OF GASTRIC OR DUODENAL ULCER | 2015 |
|
RU2595422C1 |
METHOD OF DNA EXTRACTION, SUITABLE FOR CONDUCTING QUANTITATIVE REAL-TIME PCR, FROM DRY BLOOD SPOTS OF NEWBORNS | 2017 |
|
RU2645089C1 |
METHOD FOR GENETIC MARKER PREDICTION OF RISK OF GESTOSIS | 2010 |
|
RU2431842C1 |
ENVIRONMENT AND DEVICE FOR COLLECTING, STORING AND TRANSPORTING BIOLOGICAL SAMPLES, AS WELL AS THEIR USE FOR THE STABILIZATION OF EXTRACELLULAR NUCLEIC ACIDS | 2022 |
|
RU2798013C1 |
METHOD FOR ISOLATION OF INTESTINAL VIRUS RNA FOR ANALYSIS BY RT-PCR METHOD | 2006 |
|
RU2313792C1 |
OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR IDENTIFYING GENOME OF SHEEP NAIROBI DISEASE VIRUS BY REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION | 2009 |
|
RU2416647C1 |
Authors
Dates
2020-11-12—Published
2018-12-28—Filed