METHOD OF ANALYZING MATRIX NUCLEIC ACID, METHOD FOR ANALYZING TARGET SUBSTANCE, KIT FOR ANALYZING MATRIX NUCLEIC ACID OR TARGET SUBSTANCE AND ANALYZER FOR MATRIX NUCLEIC ACID OR TARGET SUBSTANCE Russian patent published in 2021 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of analyzing a matrix nucleic acid, a method for analyzing a target substance, a kit for analyzing a matrix nucleic acid or target substance and an analyzer for a matrix nucleic acid or target substance, which have high accuracy. Invention discloses a method, where the nucleic acid amplification reagent contains a set of primers, which amplify a target sequence and a complementary sequence, and a signal-generating substance which generates or quenches the signal in response to amplification, and the signal-generating substance generates a signal in a state in which it is associated depending on the sequence, and quenches the signal in a state in which it is not associated, or quenches the signal in a state, in which it is connected depending on sequence, and generates signal in state, in which it is not connected, and generation and suppression of signal are reversible.

EFFECT: method of analyzing a matrix nucleic acid, a method for analyzing a target substance, kit for analyzing matrix nucleic acid or target substance and analyzer for matrix nucleic acid or target substance.

15 cl, 7 dwg, 11 tbl, 3 ex