FIELD: biotechnology.
SUBSTANCE: invention relates to the production of cryopreserved mononuclear cells for the full or part of the course of prevention and treatment of graft-versus-host reactions or rejection during transplantation of allogeneic organs or cutaneous T-cell lymphomas, as well as a number of autoimmune diseases. The method includes the isolation of a concentrate of mononuclear cells with induced apoptosis and carrying out extracorporeal photopheresis. Then the concentrate of mononuclear cells is divided into doses, subjected to program freezing to -70 ° C, followed by cryopreservation in liquid nitrogen for storage during the course of therapy.
EFFECT: invention allows long-term storage, as well as ability to control the process of apoptosis of mononuclear cells.
1 cl, 3 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR INDUCTION OF ANTIGEN-ASSOCIATED IMMUNE RESPONSE | 2021 |
|
RU2752967C1 |
| LOW-VOLUME METHOD FOR EXTRACORPOREAL PHOTOCHEMOTHERAPY | 2021 |
|
RU2791659C1 |
| METHOD FOR PREVENTION AND TREATMENT OF RENAL GRAFT REJECTION | 2013 |
|
RU2508924C1 |
| CONTAINER FOR DIVIDING INTO DOSES, STORAGE AND CLINICAL USE OF BLOOD COMPONENTS, CELL SUSPENSIONS AND LIQUIDS (OPTIONS) | 2023 |
|
RU2801095C1 |
| METHOD FOR TRANSPLANTATION OF PERIPHERAL BLOOD AUTOLOGOUS HAEMOPOIETIC STEM CELLS | 2019 |
|
RU2734121C1 |
| METHOD OF QUANTITATIVE ESTIMATION OF APOPTOSIS MODIFIED BY ORGANIC LOW-MOLECULAR COMPOUNDS | 2011 |
|
RU2471190C1 |
| METHOD OF ESTIMATION OF INFLUENCE OF NITROSAMINES ON APOPTOSIS IN CHILDREN LIVING IN ADVERSE ENVIRONMENTAL CONDITIONS | 2016 |
|
RU2626516C1 |
| METHOD FOR EVALUATION OF STRONTIUM IMPACT ON APOPTOSIS, ASSOCIATED WITH ALLELLIC VARIANTS OF FAS GENE IN CHILDREN | 2016 |
|
RU2621155C1 |
| COMBINATION CRYOPROTECTOR "DIMETHYL SULPHOXIDE/RHEOPOLYGLUCIN" FOR STEM CELL CRYOPRESERVATION AND METHOD FOR CRYOPRESERVATION THEREOF FOR CLINICAL APPLICATIONS | 2014 |
|
RU2563117C1 |
| METHOD FOR UNSTRUCTURED FREEZING PERIPHERAL BLOOD PRECURSOR CELLS AT MINUS 80 °C WITH A HEMICOLITE SOLUTION | 2019 |
|
RU2711152C1 |
