METHOD OF ESTIMATION OF INFLUENCE OF NITROSAMINES ON APOPTOSIS IN CHILDREN LIVING IN ADVERSE ENVIRONMENTAL CONDITIONS Russian patent published in 2017 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: blood sample is taken from the child and the presence of nitrosamine-nitroso dimethylamine (NDMA) is determined in the sample, in those blood samples of patients with NDMA, mononuclear cells are extracted and the amount of p53 protein is determined using flow cytometry. A sample of buccal epithelium is also taken from this child and deoxyribonucleic acid (DNA) is extracted from this sample. Then, genotyping of the polymorphism is carried out on the detecting polymerizer using PCR using the Arg72Pro (rs1042522) gene of the TP53 gene as a primer, by examining its allelic state, establishing for the TP53 gene one of the following conditions: heterozygous or normal homozygous or pathological Homozygous. And if the presence of pathological homozygous or heterozygous genotypes of Arg72Pro of the TP53 gene is simultaneously established, a decrease in blood content of p53 in 1.5 and more times, relative to the lower limit of the physiological norm for children. In the presence of NDMA in the blood, the process of apoptosis in a child under the influence of nitrosamines is assessed as delayed.

EFFECT: invention provides an accurate estimate of the effect of nitrosamines on apoptosis with the possibility of subsequently judging the development of immunodeficient and immunoproliferative states of the population already at an early stage.

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