FIELD: biotechnology.
SUBSTANCE: described is a method for detecting Mammarenavirus guanaritoense virus RNA by real-time RT-PCR, involving extraction of RNA from biological samples, followed by a two-step reaction – reverse transcription and polymerase chain reaction for the target RNA fragments of the genome of Mammarenavirus guanaritoense, using a set of primers and a probe, complementary to a region of the protein L gene coding RNA-dependent RNA polymerase.
EFFECT: development of a highly sensitive method for identifying Mammarenavirus guanaritoense virus RNA in biological samples and other virus-containing samples.
1 cl, 2 dwg, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD FOR DETECTING MAMMARENAVIRUS GUANARITOENSE VIRUS BY DETECTR WITH ISOTHERMAL AMPLIFICATION | 2024 |
|
RU2835994C1 |
| METHOD FOR DETECTING BANDAVIRUS DABIEENSE (SFTSV) VIRUS RNA BY REAL-TIME RT-PCR | 2024 |
|
RU2831410C1 |
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD FOR DETECTING RNA OF MAMMARENAVIRUS GUANARITOENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION | 2024 |
|
RU2834909C1 |
| METHOD OF DETECTING RNA OF VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER USING REAL-TIME RT-PCR | 2024 |
|
RU2834908C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| METHOD OF DETECTING NIPAH VIRUS USING REAL-TIME RT-PCR | 2022 |
|
RU2816270C2 |
| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
RU2744198C1 |
| METHOD OF DETECTING GUANARITO VIRUS BY METHOD BASED ON USE OF DEOXYRIBOZYME 10-23 | 2024 |
|
RU2827922C1 |
