FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology and includes methods and compositions for elimination of polyomaviruses, such as John Cunningham virus, from host cells and treatment of diseases associated with polyomavirus, such as progressive multifocal leukoencephalopathy (PML). These compositions include isolated nucleic acid sequences containing CRISPR-associated endonuclease and an RNA conductor, wherein the RNA conductor is complementary to the target sequence in the polyomavirus.
EFFECT: invention provides methods and compositions for elimination of polyomaviruses, such as John Cunningham virus, from host cells and treatment of diseases associated with polyomavirus, such as progressive multifocal leukoencephalopathy (PML)
24 cl, 9 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| GENOMIC DNA EDITING SYSTEM OF AN EUKARYOTIC CELL BASED ON THE NUCLEOTIDE SEQUENCE ENCODING THE SUCAS9NLS PROTEIN | 2022 |
|
RU2804422C1 |
| PaCas9 NUCLEASE | 2018 |
|
RU2706298C1 |
| CRISPR-CAS SYSTEMS AND METHODS OF CHANGING EXPRESSION OF GENE PRODUCTS | 2013 |
|
RU2687451C1 |
| CRISPR-CAS SYSTEMS AND METHODS FOR CHANGING THE EXPRESSION OF GENE PRODUCTS | 2013 |
|
RU2796273C2 |
| METHOD FOR CREATION OF T-CELLS SUITABLE FOR ALLOGENEIC TRANSPLANTATION | 2015 |
|
RU2752933C2 |
| GENE EDITING USING MODIFIED CLOSED-END DNA (ceDNA) | 2018 |
|
RU2811724C2 |
| RNA-CONDUCTORS TO SUPPRESS THE REPLICATION OF HEPATITIS B VIRUS AND FOR THE ELIMINATION OF HEPATITIS B VIRUS FROM HOST CELL | 2017 |
|
RU2652899C1 |
| RECOMBINANT PLASMID DNAS PX458D10A_MYC, PROVIDING MYC GENE KNOCKOUT IN HUMAN CELLS, METHOD OF PRODUCING HUMAN CELL LINE WITH MYC GENE KNOCKOUT AND HUMAN BREAST CANCER CELL LINE MDA-MB231_MYC WITH SUPPRESSED MYC GENE EXPRESSION | 2022 |
|
RU2818348C1 |
| DELIVERY AND USE OF SYSTEMS OF CRISPR-CAS, VECTORS AND COMPOSITIONS FOR TARGETED ACTION AND THERAPY IN LIVER | 2014 |
|
RU2716420C2 |
| METHODS AND COMPOSITIONS FOR TREATING HUNTINGTON'S DISEASE | 2015 |
|
RU2691102C2 |
