FIELD: medicine.
SUBSTANCE: inventions group relates to medicine, namely to tissue engineering, regenerative medicine, traumatology and orthopedics, and can be used to restore bone tissue in the area of a bone defect. The method includes: installing a temporary insert-spacer corresponding to the size of the defect in the area of the bone defect; removal of the temporary spacer insert is performed 7-30 days after installation; installation of a perforated resorbable membrane with adhered cellular spheroids from the autologous periosteum deposited on its surface. In this case, the perforated membrane is folded in the form of a cylinder and is attached to the periosteum of the ends of the bone sawdust using surgical sutures. In special cases, the temporary spacer insert is made of polymethyl methacrylate, the resorbable membrane is made of a biodegradable material, the biodegradable material is selected from an allogeneic lyophilized periosteum. At the same time, when installing a perforated membrane with cellular spheroids, it is fixed at the edges with suture absorbable threads, the subject is a human, the bone defect is a defect in the tubular bone, a temporary spacer insert is installed in the form of a cylinder, and the cavity inside the membrane is filled with alginate gel. The resorbable membrane is collagen barrier membrane, holes of which have a diameter of 150-350 microns, the distance between the centers of the holes is 550-1000 microns and can be 10-50% of the total surface area of the entire membrane.
EFFECT: invention provides the ability to restore bone tissue in the area of a bone defect by growing bone regenerate in the area of the de novo de novo on a perforated resorbable membrane with adhered cell spheroids from the autologous periosteum deposited on its surface.
14 cl, 1 ex
