FIELD: medicine.
SUBSTANCE: invention refers to medicine, namely to tissue engineering, regenerative medicine, orthobiology, traumatology and orthopaedics, otolaryngology, plastic and reconstructive surgery. Disclosed is a method for producing a tissue-engineered periosteum from cell spheroids for the restoration of bone defects in patients, characterized by that the collagen membrane is placed at the bottom of the culture vessel with a side cover and is impregnated with the culture medium. Further, a rectangular titanium frame with cross-sectional size of 4×4 with frame height of 0.5 mm is placed on top of the collagen membrane. Frame is used to press the edges of the collagen membrane to the bottom of the flask; a horizontal layer of cell spheroids consisting of aggregated cultured autologous periosteal cells is laid on top of the collagen membrane bounded by the inner walls of the frame. Then spheroids are cultivated on the surface of the collagen membrane during maturation of the tissue-engineered structure in a nutrient medium: DMEM or DMEM/F12 450 ml, L-glutamine 292 mg, thrombolysate 50 ml, penicillin 100 units/ml, streptomycin 100 mcg/ml at 100% humidity and with 5% content of CO2 in the air mixture above the layer of the nutrient medium inside the culture flask, for 6-7 days with replacement of the culture medium at least 2 times.
EFFECT: effective engraftment and regeneration of skeletal tissues is achieved.
5 cl, 1 dwg, 3 ex
