FIELD: medicine.
SUBSTANCE: invention relates to the field of medicine and medical equipment, namely, to experimental traumatology and orthopaedics, regenerative medicine. Liquid in vivo bioreactor for growing bone tissue between bone fragments in case of segmental defects of long bones exceeding the critical size is made in the form of a round hollow pipe made of polymethyl methacrylate bone cement, the pipe has thickenings of the same material at the edges of both pipe openings, funnels made of bioinert natural latex are set at the ends of the pipe on two sides; the edge of the pipe is passed into the hole of the hollow cone of the funnel with a larger-radius base, and the end of the bone fragment is passed into the free opening of the funnel tube; the ends of the proximal and distal bone fragments are located opposite each other inside the pipe of the in vivo bioreactor; funnel cuffs made of the same bioinert natural latex and facing the inside of the funnel lumen, soldered non-detachably with a truncated cone, are located at the ends of the hollow cones of the funnels; two additional holes located opposite each other are provided in the inner wall of the pipe of the in vivo bioreactor, whereto the lumens of the tubes made of medical silicone rubber and the titanium screw-in metal fitting are opened, wherein said fitting is connected with the end of one of the tubes made of medical silicone rubber; the two tubes made of medical silicone rubber via a metal fitting are connected with infusion adapters having direct-flow channels for LUER lock-type sealed connection with the syringe attached for replacing the solutions; part of one of the tubes made of medical silicone rubber is located on the inside of the wall of the methyl methacrylate pipe of the in vivo bioreactor and extends to the opposite inner hole in the pipe wall; the infusion adapters have protective caps. The cavity of the in vivo bioreactor is a controlled internal space of the apparatus, limited by the inner walls of the pipe, funnels, and bone fragments, filled with a known composition - a nutrient cell cultivation medium, namely DMEM medium with glutamine with the addition of a penicillin-streptomycin mixture, 10% rabbit platelet lysate, and recombinant human vascular endothelial growth factor A, isoform 165 (rhVEGF-A165) in an amount of 0.25 ng per 1 ml of medium applied to create a local liquid medium favourable for reparative osteogenesis and stimulation of the growth of bone regenerates from the proximal and distal bone fragments towards each other until fusion thereof into a whole bone; wherein the bone fragments with edges inside the apparatus are stabilised along the longitudinal axis of the bone by means of an external fixation apparatus for external transosseous osteosynthesis.
EFFECT: invention increases the efficiency of growing bone tissue by means of an in vivo bioreactor by growing bone structures in a favourable liquid medium with nutrients and biostimulants at the regular place for the bone (orthotopically), as well as expands the indications for applying orthotopically located in vivo bioreactors.
3 cl, 1 dwg, 1 ex
