FIELD: medicine.
SUBSTANCE: invention relates to medicine, in particular to experimental pharmacology, and can be used to increase the survival rate of cell cultures. A method for increasing the survival of human endothelial cells is proposed, including modeling oxidative stress by adding a peptide with the amino acid sequence UEHLERALNSS or a peptide with the amino acid sequence UEQLERALNTS.
EFFECT: modeling oxidative stress in primary culture of human endothelial cells (HUVEC) by adding hydrogen peroxide H2O2 (200 mcM) led to significant cell death. Preliminary addition to the culture medium of a peptide with the amino acid sequence UEHLERALNSS (sample EP-11-1) or a peptide with the amino acid sequence UEQLERALNTS (sample EP-11-3) significantly increased the number of surviving cells under conditions of oxidative stress.
1 cl, 1 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR REDUCING AGGREGATION ABILITY OF PLATELETS | 2021 |
|
RU2751964C1 |
| METHOD OF CORRECTION OF STRUCTURAL DAMAGES IN PLACENTA WITH PEPTIDE IMITATING ALPHA-HELIX B ERYTHROPOIETIN IN ADMA-LIKE MODEL OF PREECLAMPSIA | 2020 |
|
RU2724402C1 |
| METHOD FOR CORRECTING MICROCIRCULATION DISORDERS IN KIDNEY WITH PEPTIDE THAT MIMICS ALPHA-HELIX B OF ERYTHROPOIETIN IN ISCHEMIA-REPERFUSION INJURY | 2021 |
|
RU2751413C1 |
| RECOMBINANT GENE ENCODING HBD-EPO PROTEIN, RECOMBINANT PLASMID DNA PL610, METHOD FOR PRODUCING A RECOMBINANT HBD-EPO PROTEIN, A RECOMBINANT HBD-EPO PROTEIN, A COMPOSITION FOR SPECIFICALLY INDUCING BONE REGENERATION, METHOD OF SPECIFIC INDUCTION OF BONE TISSUE REGENERATION | 2017 |
|
RU2664192C1 |
| METHOD FOR PREVENTING MICROCIRCULATION DISORDERS IN KIDNEYS WITH ASIALISED ERYTHROPOIETIN IN EXPERIMENT | 2018 |
|
RU2696864C1 |
| METHOD OF PLACENTAL MICROCIRCULATION CORRECTION BY PEPTIDE IMITATING B ERYTHROPOIETIN ALPHA-SPIRAL WITH ADMA-LIKE PRE-ECLAMPSIA MODEL | 2020 |
|
RU2731919C1 |
| METHOD OF ENDOTHELIAL DYSFUNCTION CORRECTION BY A PEPTIDE IMITATING ALPHA-HELIX B ERYTHROPOIETIN, WITH AN ADMA-LIKE MODEL OF PREECLAMPSIA | 2020 |
|
RU2732107C1 |
| METHOD FOR CORRECTION OF MICROCIRCULATORY DISORDERS OF THE RETINA WITH LOW-SIALATED ERYTHROPOIETIN | 2021 |
|
RU2750966C1 |
| METHOD OF CORRECTION OF MICROCIRCULATION DISORDERS IN PLACENTA WITH CARBAMYLATED DARBEPOETIN IN ADMA-LIKE MODEL OF PREECLAMPSIA | 2019 |
|
RU2720103C1 |
| METHOD FOR CORRECTING MICROCIRCULATORY DISORDERS OF THE RETINA WITH ASIALATED ERYTHROPOIETIN | 2021 |
|
RU2762892C1 |
