FIELD: molecular biology.
SUBSTANCE: invention is aimed to determine the spatial structure of biological oligonucleotide molecules, such as DNA/RNA aptamers. The essence of the invention consists in measuring small-angle X-ray scattering from biomolecules in solution, determining structural parameters, measuring giant Raman scattering spectra, modeling a full-atomic model of the spatial structure of an oligonucleotide biomolecule, while constructing a full-atomic model of the structure of an oligonucleotide biomolecule, its oligonucleotide sequence is used, the most probable secondary structures based on it, separate bands of giant Raman scattering spectra unique for the molecule in the range of 600-1800 cm-1, from which a set of data on interatomic bonds in the molecule responsible for the formation of elements of secondary and tertiary structures is extracted and formed, as well as the validation of the small-angle X-ray scattering pattern from the model for compliance with the scattering pattern from a monodisperse solution with oligonucleotide molecules.
EFFECT: providing the possibility of obtaining the spatial structure of biological molecules of high-resolution oligonucleotides.
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