METHOD FOR DETERMINING QUALITATIVE PARAMETERS OF IMMUNOSUPPRESSIVE CELLS IN CANCER PATIENTS Russian patent published in 2021 - IPC G01N33/577 

FIELD: medicine.

SUBSTANCE: invention relates to medicine and is intended to determine the qualitative parameters of immunosuppressive cells of patients with breast cancer. Peripheral blood of the patient is taken. The peripheral blood sample is divided into two parts: for determining the phenotypic characteristics and the amount of receptors on the surface of cells and for testing the cytotoxic activity. Peripheral blood leukocytes are labelled with monoclonal antibodies against surface receptors CD4, CD8, TIM-3, PD-1. In case of increased release of the intracellular enzyme lactate dehydrogenase, antitumoral activity of leukocytes is determined. Reduced cytotoxic activity is determined by an increase in the relative amount of PD-1 and TIM-3-positive CD4+ and CD8+ T-cells, the absolute amount of TIM-3 positive CD4+ and CD8+ T-cells, an increase in PD-1-positive and CD8+ T-cells, and an increase in the amount of molecules per cell for TIM-3-positive CD8+ T cells and PD-1-positive CD4+ T-cells compared to cells without monoclonal antibody staining.

EFFECT: invention provides improved accuracy of determining the degree of immunosuppression in cancer patients.

2 cl, 7 dwg, 3 tbl