FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. The method includes design of genetic constructs, methods for transfection of cell lines, purification and evaluation of expression of target protein. Genetic construct pVNV-GL-RBDind is obtained, containing nucleotide sequence encoding protein RBD SARS-CoV-2 line B.1.617, and providing expression of protein of this RBD in mammalian cells and further purification using affinity chromatography. The invention can be used to obtain protein RBD - a recombinant structural domain of the spike glycoprotein SARS-CoV-2 line B.1.617.
EFFECT: resulting RBD protein is intended for the direct production of a vaccine preparation against SARS-CoV-2 of the B.1.617 line.
1 cl, 1 dwg, 1 tbl, 4 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID PVBL-RBDDELTA PROVIDING SYNTHESIS AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS LINE B.1.617.2 IN MAMMALIAN CELLS |
2021 |
- Toropovskii Andrei Nikolaevich
- Viktorov Denis Aleksandrovich
- Shcherbakov Dmitrii Nikolaevich
- Nikitin Aleksei Georgievich
|
RU2772904C1 |
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2021 |
- Viktorov Denis Aleksandrovich
- Toropovskii Andrei Nikolaevich
- Shcherbakov Dmitrii Nikolaevich
- Nikitin Aleksei Georgievich
|
RU2772905C1 |
| INTEGRATIVE PLASMID VECTOR pVEAL3-RBDdel, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT PROTEIN OF RECEPTOR-BINDING DOMAIN RBDdelta OF CORONAVIRUS SARS-CoV-2 IN MAMMALIAN CELLS, RECOMBINANT CELL LINE STRAIN CHO-K1-RBDdelta AND RECOMBINANT PROTEIN RBDdelta SARS-CoV-2 PRODUCED BY CELL LINE STRAIN |
2023 |
- Nesmeyanova Valentina Sergeevna
- Merkuleva Yuliya Aleksandrovna
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Volkova Natalya Vyacheslavovna
- Belenkaya Svetlana Valerevna
- Borgoyarkova Mariya Borisovna
- Volosnikova Ekaterina Aleksandrovna
- Esina Tatyana Igorevna
- Zajkovskaya Anna Vladimirovna
- Pyankov Oleg Viktorovich
- Ilichev Aleksandr Alekseevich
|
RU2816175C1 |
| INTEGRATIVE PLASMID VECTOR PVEAL2-S-RBD, PROVIDING THE EXPRESSION AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS IN MAMMALIAN CELLS, THE RECOMBINANT CHO-K1-RBD CELL LINE STRAIN AND THE RECOMBINANT SARS-COV-2 RBD PROTEIN PRODUCED BY THE SPECIFIED STRAIN OF THE CELL LINE CHO-K1-RBD |
2021 |
- Merkuleva Yuliya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Belenkaya Svetlana Valerevna
- Isaeva Anastasiya Aleksandrovna
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
- Volkova Natalya Vyacheslavovna
- Ilichev Aleksandr Alekseevich
- Karpenko Larisa Ivanovna
|
RU2752858C1 |
| ARTIFICIAL GENE CODING A BICISTRONIC STRUCTURE FORMED BY RECEPTOR-BINDING DOMAIN SEQUENCES OF THE GLYCOPROTEIN S OF THE SARS-COV-2 CORONAVIRUS, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
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|
RU2733831C1 |
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2020 |
- Imatdinov Ilnaz Ramisovich
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- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
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|
RU2733832C1 |
| ARTIFICIAL ECTOS_SC2 GENE ENCODING AN ECTODOMAIN OF THE SARS-COV-2 CORONAVIRUS S GLYCOPROTEIN WITH A C-TERMINAL TRIMERIZATION DOMAIN, A RECOMBINANT PLASMID PSTEM-RVSV-ECTOS_SC2, WHICH PROVIDES EXPRESSION OF THE ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-ECTOS_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
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- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2733834C1 |
| ARTIFICIAL GENETIC CONSTRUCT FOR THE HETEROLOGOUS EXPRESSION OF THE S-PROTEIN RECEPTOR-BINDING DOMAIN IN A FUSED POLYPEPTIDE CHAIN WITH A NUCLEOCAPSID PROTEIN |
2022 |
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|
RU2801597C1 |
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2020 |
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|
RU2744274C1 |
| RECOMBINANT INFLUENZA VIRUS INTENDED FOR THE PREVENTION OF COVID-19 AND INFLUENZA, METHOD OF ITS PRODUCTION AND USE |
2022 |
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