FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, namely to the creation of a new fused protein containing polyphemusine I polypeptide of L Polyphemus and modified GroEL chaperone of T.thermophilys microorganism. New protein is modified methionine-free GroEL protein, which is stable, is not subjected to proteolysis, contains, in an encoding part between Ser199 and Tyr201 codons, a polylinker, and polyphemusine I polypeptide fused to it in the polylinker area, which is highly expressed in a host cell.
EFFECT: new protein can be used in the pharmaceutical industry to obtain isolated stabled proteins, biosynthesis of which is difficult or not possible, or their physical and chemical properties do not allow one to isolate and obtain synthesized proteins in the purified and active form.
1 cl, 3 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MODIFIED GROEL PROTEIN OF MICROORGANISM THERMUS THERMOPHILUS | 2018 |
|
RU2740715C2 |
| GROEL PROTEIN OF MICROORGANISM THERMUS THERMOPHILUS WITH ALTERED AMINO ACID SEQUENCE | 2018 |
|
RU2731895C2 |
| FUSION PROTEIN, ESCHERICHIA COLI STRAIN BEING FUSION PROTEIN PRODUCER AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2b OF SUCH FUSION PROTEIN | 2010 |
|
RU2441072C1 |
| HYBRID PROTEIN (VERSIONS), BACTERIAL STRAIN ESCHERICHIA COLI - HYBRID PROTEIN PRODUCER (VERSIONS) AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2 | 2011 |
|
RU2453604C1 |
| METHOD FOR PRODUCING OF HUMAN METHIONINE-FREE INTERFERON-ALPHA2b | 2009 |
|
RU2432401C2 |
| GENETIC DESIGN FOR EXPRESSION OF THE FUNCTIONALLY-ACTIVE HUMAN STRESS-PROTEIN (HPS70) WITH MUTATED GLYCOSILATION SITES FOR WORKING IN EUCARIOTIC EXPRESSION SYSTEMS | 2016 |
|
RU2647570C1 |
| RECOMBINANT PLASMID pET-15b_T1_RL DNA PROVIDING SYNTHESIS OF RECOMBINANT FUSION PROTEIN CONSISTING OF TUMOR-SPECIFIC PEPTIDES AND ANTITUMOR PEPTIDE RL2, AND RECOMBINANT FUSION PROTEIN POSSESSING CYTOTOXIC ACTIVITY AGAINST CANCER CELLS AND TARGETED PROPERTIES AGAINST TUMOR TISSUE | 2016 |
|
RU2619050C1 |
| RECOMBINANT PLASMID DNA pET22b(+)/"вд"-LINKS 1, CODING PROTEIN WITH LINKS 1 PROPERTIES, AND STRAIN OF Escherichia coli BL21 (DE3)/pET22b(+)/"вд"-LINKS 1 - PRODUCENT OF PROTEIN WITH PROPERTIES OF HUMAM LINKS 1 | 2009 |
|
RU2404246C1 |
| RECOMBINANT PLASMID DNA pET22b(+)/SLURP-1 CODING SLURP-1 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET22b(+)/SLURP-L-PRODUCENT OF HUMAN SLURP-1 PROTEIN | 2010 |
|
RU2453602C2 |
| RECOMBINANT PLASMID pET-15b_T3_RL DNA PROVIDING SYNTHESIS OF RECOMBINANT FUSION PROTEIN CONSISTING OF TUMOR-SPECIFIC PEPTIDES AND ANTITUMOR PEPTIDE RL2, AND RECOMBINANT FUSION PROTEIN POSSESSING ANTI-TUMOR ACTIVITY AGAINST HUMAN BREAST CANCER | 2016 |
|
RU2619053C1 |
