FIELD: biotechnology.
SUBSTANCE: group of inventions relates to a live recombinant strain of Mycobacterium bovis-BCG for protection against tuberculosis and to a use thereof. Proposed is a live recombinant strain of Mycobacterium bovis-BCG for protection against tuberculosis, overexpressing nucleic acids encoding the PhoP and PhoR proteins, wherein the overexpression is executed by methods for genetic engineering. The described strain is used as part of a pharmaceutical composition and a vaccine intended for treatment or prevention of infection with Mycobacterium tuberculosis mycobacteria in a mammal. The described strain is used in a method for treating or preventing infection with Mycobacterium tuberculosis in a mammal, as well as in a method for treating or preventing mammalian bladder cancer.
EFFECT: group of inventions provides a possibility of obtaining a strain with enhanced immunogenicity and protective efficiency, as well as moderate virulence.
16 cl, 7 dwg, 6 ex
