METHODS FOR OBTAINING SINGLE-STRANDED RNA Russian patent published in 2021 - IPC C12N15/10 

FIELD: biotechnology.

SUBSTANCE: group of inventions is described, including a method for obtaining purified single-stranded RNA (hereinafter – ssRNA) (options). In one implementation, the method includes stages of (i) producing RNA preparation containing ssRNA using in vitro transcription, (ii) bringing RNA preparation into contact with cellulose material under conditions that provide for the possibility of binding double-stranded RNA (hereinafter – dsRNA) with cellulose material and do not allow ssRNA to bind with cellulose material; and (iii) separating ssRNA from cellulose material under conditions that provide for the possibility of binding dsRNA with cellulose material and do not allow ssRNA to bind with cellulose material.

EFFECT: invention expands the arsenal of methods for preparing purified single-stranded RNA.

39 cl, 11 dwg, 11 ex