FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology; it can be used in medicine, namely for the use in vaccine prophylaxis and vaccine therapy using induction of CTL immune response. An expression vector containing polynucleotide encoding a fused protein is claimed. The specified fused protein contains (i) human HIV-1 Nef protein with mutations 3C, 153L and 177G, and it is fused on its C-end with (ii) an immunogenic antigen. A vaccine composition is proposed, including an expression vector and one or several pharmaceutically acceptable fillers and/or adjuvants. A method for the induction of CTL immune response in a subject is proposed, where the method includes injection to a subject of an expression vector.
EFFECT: invention allows for creating a vaccine based on the formation of endogen constructed exosomes that is capable of inducing CT immune response against infectious and tumor diseases.
12 cl, 28 dwg, 1 tbl, 3 ex
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