BIOCHIP INCLUDING SEQUENCING PRIMER AND NON-SEQUENCING UNIT Russian patent published in 2022 - IPC B01J19/00 C12Q1/68 G01N33/48 

FIELD: biochemistry.

SUBSTANCE: biochip for sequencing DNA or RNA nucleic acids is disclosed, including: substrate including a set of discrete holes; gel-like material placed in each of discrete holes; a sequencing primer grafted on gel-like material; and a non-sequencing unit grafted on gel-like material and connected by means of a linker to a triplet state damper, antioxidant or a donor for fluorescent resonance energy transfer (FRET); where both the sequencing primer and the non-sequencing unit are in the form, in which they were grafted, where the non-sequencing unit is polyethylene glycol or PolyT, and where the sequencing primer and the non-sequencing unit are grafted by the exposure to substrate of a solution or solutions, in which a molar ratio of the non-sequencing unit to the sequencing primer is from 0.25:1 to 10:1. A method for producing the specified biochip is also disclosed.

EFFECT: group of inventions, due to the fact that the non-sequencing unit serves as a spacer between sequencing primers, provides the spatial separation of sequencing primers, which improves the amplification due to the limiting of steric complications in proteins involved in the amplification process.

15 cl, 12 dwg, 3 tbl, 3 ex