FIELD: biochemistry.
SUBSTANCE: invention relates to an expression cassette of a polypeptide toxic to coleopteran species Diabrotica virgifera virgifera (western corn rootworm), Diabrotica barberi (northern corn rootworm) or Diabrotica undecimpunctata howardi (southern corn rootworm). Also disclosed is a polypeptide; an expression vector and a host cell comprising said expression cassette; transgenic plant containing said expression cassette; composition containing said polypeptide. Disclosed are methods of producing said polypeptide; obtaining a plant or part of a plant; increasing plant resistance to coleoptera species Diabrotica virgifera virgifera (western corn rootworm), Diabrotica barberi (northern corn rootworm) or Diabrotica undecimpunctata howardi (southern corn rootworm); identification of a plant or part of a plant; killing or controlling a population of coleopteran pests of the species Diabrotica virgifera virgifera (western corn rootworm), Diabrotica barberi (northern corn rootworm) or Diabrotica undecimpunctata howardi (southern corn rootworm); higher yield.
EFFECT: invention has the ability to effectively control coleopteran pests.
36 cl, 10 ex, 8 tbl
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