FIELD: biotechnology.
SUBSTANCE: disclosed is a method of determining corrected color values from image data obtained during a base recognition cycle using a sequencer and a nucleic acid sequencer. Method comprises (a) imaging a substrate comprising a plurality of regions on which nucleic acid bases are read, wherein these areas have colors representing types of nucleic bases; (b) measuring color values of a plurality of areas on said image of the substrate; (c) storing the color values in a processor buffer of one or more sequencer processors; (d) extracting color values with partial phasing of a plurality of areas, where said color values with partial phasing correction were stored in memory of sequencer during implementation of immediately preceding cycle of base recognition; (e) determining the advance phasing correction from the color values with partial phasing correction stored during the immediately preceding cycle of base recognition, and color values stored in the processor buffer; and (f) determining the corrected color values from the color values in the processor buffer, the color values with partial phasing, stored during the immediately preceding cycle, and correcting the advanced phasing. Sequencer comprises an image acquisition system, a memory device and processors configured to perform said method actions.
EFFECT: invention reduces the load on the storage device.
39 cl, 11 dwg
