METHODS AND SYSTEMS FOR OBTAINING SETS OF UNIQUE MOLECULAR INDICES WITH HETEROGENEOUS LENGTH OF MOLECULES AND CORRECTING ERRORS THEREIN Russian patent published in 2022 - IPC C12Q1/6855 C12Q1/6869 G16B25/00 G16B30/00 

FIELD: biotechnology.

SUBSTANCE: disclosed is a method of sequencing nucleic acid molecules (versions), a method of producing adapters for sequencing, a computer system and a computer product for implementing said methods. Sequencing methods include obtaining a DNA adapter, where each adapter contains a non-random unique molecular index, wherein non-random unique molecular indices of adapters form a set of non-random unique molecular indices with variable length, amplification of DNA adapter, sequencing a plurality of amplified polynucleotides to obtain a plurality of reads associated with said set, identification among a plurality of reads associated with the same set of reads, obtaining set alignment values for each read. Method of producing adapters involves providing a set of sequences of oligonucleotides with different lengths of molecules, selecting a subset of the oligonucleotide sequences from the set, where all the editing distances between the oligonucleotide sequences of the subset correspond to a threshold value, and a subset of sequences of oligonucleotides forms a set of non-random unique molecular indexes with variable length, synthesis of multiple adapters for sequencing.

EFFECT: inventions provide unambiguous identification of each initial DNA molecule during the sequencing process.

43 cl, 2 ex, 3 tbl, 19 dwg