SYSTEMS AND APPARATUSES FOR SEQUENCING WITH HIGH THROUGHPUT CAPACITY WITH DETECTION BASED ON SEMICONDUCTORS Russian patent published in 2021 - IPC G01N21/64 

FIELD: chemistry.

SUBSTANCE: invention relates to biological or chemical analysis, namely to systems and methods using detection apparatuses for biological or chemical analysis. The apparatus for determining nucleotide bases in a nucleic acid sequence comprises a biosensor and a receiver configured to hold the biosensor, wherein the biosensor has a surface for samples, containing pixel areas and containing multiple clusters during the sequence of events of analysing samples so that the clusters are distributed unevenly over the pixel areas, a matrix of sensors, wherein each sensor in the matrix is configured to read information from one or more clusters located in the corresponding pixel areas of the surface for samples to generate a pixel signal in a sample analysis event, wherein the matrix is configured to generate a plurality of pixel signal sequences, the matrix has N active sensors, the sensors in the matrix are arranged relative to the surface for samples so as to generate the corresponding pixel signals during the sequence of events of analysing samples from N corresponding pixel areas of the surface for samples to generate a plurality of pixel signal sequences, and a communication port configured to output a plurality of pixel signal sequences; and a signal processor connected with the receiver and configured to analyse the time sequence and the space of the plurality of pixel signal sequences to detect illumination patterns of corresponding N+M individual clusters on the surface for samples from N active sensors, wherein M is a positive integer, and to classify the results of the sequence of events of analysing a sample for N+M individual clusters, and configured to use the plurality of sequences of pixel signals taken from the group of pixel areas, wherein at least two clusters are unevenly distributed over said areas, to determine the spatial characteristics of said at least two clusters, including the position of said at least two clusters on the surface for samples, wherein the plurality of pixel signal sequences encodes differential interference between said at least two clusters, caused by uneven distribution thereof over the groups of pixel areas.

EFFECT: increased throughput capacity of solid-state imaging systems.

17 cl, 17 dwg