FIELD: biotechnology.
SUBSTANCE: invention relates to a genetic construct for expression of foreign genes in cells of insect Polypedilum vanderplanki in conditions of high radiation background. Disclosed genetic construct includes a gene isolated from the DNA sequence of Polypedilum vanderplanki, coding the PDiap1 protein, with the nucleotide sequence SEQ ID NO: 1, pGAPDH promoter, polyadenylation site, PUC replication origin and antibiotic kanamycin resistance gene.
EFFECT: invention provides higher radiation resistance of insect Polypedilum vanderplanki cells.
1 cl, 4 dwg, 1 tbl, 1 ex
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Authors
Dates
2022-03-16—Published
2021-03-29—Filed