METHOD FOR PREDICTING THE CLINICAL COURSE OF NEUROENDOCRINE NEOPLASMS OF THE COLON Russian patent published in 2022 - IPC G01N33/574 C12Q1/68 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to the field of genetics and molecular oncology, and concerns a method for predicting the clinical course of neuroendocrine neoplasms of the colon. The method includes the selection of biopsy material, isolation of total DNA from tissue samples using the method for phenol-chloroform extraction, bisulfite conversion of DNA, followed by its use as a matrix for amplification of regulatory regions in the presence of gene-specific primers, pyrosequencing of the obtained amplicons in the presence of a specific primer, determination of methylation of the promoter region of the gene. If the methylation status of Met_LINE1 is more than 72%, Met_APC1 is less than 11%, Met_MGMT is less than 10.5%, Met_RASSF1A is less than 12% and Met_RUNX3 is less than 10%, a favorable clinical course of the disease should be expected, with Me_tLINE values less than or equal to 72%, Met_APC1 is greater than or equal to 11%, Met_MGMT is greater than or equal to 10.5%, Met_RASSF1A is greater than or equal to 12% and Met_RUNX3 is greater than or equal to 10%, on the contrary, an unfavorable course of the disease should be expected.

EFFECT: invention makes it possible to distinguish two categories of patients at the preoperative stage on the basis of a favorable and unfavorable prognosis of the clinical course of the disease and to determine the rational tactics of patient management.

1 cl, 2 ex