METHOD FOR OBTAINING BIOINK THAT PROVIDES A HIGH LEVEL OF POROSITY IN TISSUE ENGINEERIED STRUCTURES Russian patent published in 2022 - IPC A61L27/38 B29C64/106 C12N5/71 

FIELD: tissue engineering.

SUBSTANCE: invention relates to tissue engineering and 3D bioprinting. The method reveals the production of bioink, which ensures the formation of pores in a tissue-engineered structure. The method involves the preparation of bioink, which is prepared immediately before the 3D bioprinting process, using two components: a hydrogel carrying a cell culture and a water-soluble powdery component. The powdery component is presented in the form of dry lyophilized granules, their diameter does not exceed the diameter of the bioprinter nozzle. After the bioprinting process, the tissue-engineered structure is filled with a nutrient medium or buffer solution.

EFFECT: method makes it possible to obtain bioink with a high level of porosity, which can be used to create tissue-engineered structures where the cellular component requires a high level of access of nutrients and gases, without the use of additional supporting hydrogels during production.

1 cl, 7 dwg, 1 tbl, 1 ex