FIELD: biotechnology.
SUBSTANCE: group of inventions relates to tools for optimising detection of genome changes in live cells. Disclosed is a unit for a module for solid-wall cell isolation, substantial isolation, growth, editing induction, normalization and selection (SWIIN), intended for cell isolation, cell growth, creation of conditions for editing cells, and normalization or selection of small cell colonies, containing a retaining element, a perforated element, a filter, a seal surrounding the perforated element and the filter; a permeable element, and means for connecting the retaining element, the perforated element, the filter, the seal, and the permeable element. Also disclosed are SWIIN modules.
EFFECT: group of inventions ensures automated highly productive and extremely sensitive screening for identifying edited cells.
20 cl, 78 dwg, 6 tbl, 15 ex
| Title | Year | Author | Number |
|---|---|---|---|
| AUTOMATED EQUIPMENT FOR OBTAINING LIBRARIES OF PEPTIDES OF T-CELL RECEPTORS | 2019 |
|
RU2770700C1 |
| AUTOMATED MULTI-MODULE TOOL FOR EDITING CELLS (OPTIONS) | 2018 |
|
RU2755308C2 |
| TRANSGENIC ANIMALS AND TRANSGENIC EMBRYOS PRODUCING ENGINEERED NUCLEASE | 2019 |
|
RU2817017C2 |
| PaCas9 NUCLEASE | 2018 |
|
RU2706298C1 |
| NUCLEASE Cpf1 FROM BACTERIUM RUMINOCOCCUS BROMII, DNA MOLECULE OR RNA CODING NUCLEASE, VECTOR CONTAINING SAID DNA MOLECULE, CRISPR/Cpf1 SYSTEM CONTAINING SAID NUCLEASE AND GUIDE RNA, HOST CELL FOR PRODUCING NUCLEASE Cpf1, METHOD OF PRODUCING NUCLEASE Cpf1 AND USE THEREOF | 2022 |
|
RU2816876C1 |
| MATERIALS AND METHODS FOR CANCER TREATMENT | 2019 |
|
RU2787828C2 |
| GENOMIC DNA EDITING SYSTEM OF AN EUKARYOTIC CELL BASED ON THE NUCLEOTIDE SEQUENCE ENCODING THE SUCAS9NLS PROTEIN | 2022 |
|
RU2804422C1 |
| COMPOSITIONS AND METHODS FOR TREATMENT OF HEMOGLOBINOPATHIES | 2013 |
|
RU2650811C2 |
| METHODS AND PRODUCTS FOR EXPRESSION OF PROTEINS IN CELLS | 2013 |
|
RU2711249C2 |
| METHODS AND COMPOSITIONS FOR MODIFYING TARGET LOCUS | 2015 |
|
RU2704283C2 |
