METHOD FOR PRODUCING OXIDASES OF THE MICROTHIELAVIA OVISPORA VKM F-1735 FUNGUS THAT DECOLOR INDUSTRIAL DYES WITHOUT A MEDIATOR Russian patent published in 2022 - IPC C12N1/14 C12N9/02 C12N9/30 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular to a method for obtaining oxidases of the fungus Microthielavia ovispora VKM F-1735, the ability of oxidases of the culture liquid of the specified strain to decolorize or modify the color of industrial dyes without the use of redox mediators is shown. It is possible to use oxidases produced by the fungus strain by the proposed method in technologies used in the food and textile industries, fish farms, wastewater treatment, cosmetology, the creation of biosensors and test systems without additional costs associated with the use of redox mediators. Submerged cultivation of the fungus Microthielavia ovispora VKM F-1735 is carried out in a mineral medium with the addition of crushed pea seeds as a natural source of carbon and energy until the maximum laccase activity in the culture liquid is obtained, the resulting culture liquid of the fungus is separated from the mycelium. Oxidases of the obtained culture liquid of the fungus Microthielavia ovispora VKM F-1735 are capable of bleaching or modifying, followed by a change in color or intensity of a wide range of industrial dyes at concentrations up to 670 mcM without additional use of mediators, which reduces the cost of technological processes. For the first time, for representatives of the genera Microthielavia and Thielavia, data are presented on the possibility of bleaching and color modification of industrial dyes by oxidases with the culture liquid of the fungus Microthielavia ovispora VKM F-1735, and the effect of various environmental conditions on the laccase activity of the obtained preparation is shown.

EFFECT: ability of oxidases of the cultural liquid of the fungus obtained by this method to carry out the complete degradation of malachite green at concentrations up to 134 mcM in a liquid medium.

1 cl, 5 dwg, 1 tbl