FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used in medical and agricultural microbiology, veterinary medicine. E. coli cultures isolated from sick and/or fallen farm animals, as well as from litter, feed, drinking systems, abiotic surfaces of milking equipment are identified by bacteriological method. Additionally, they are subjected to molecular genetic analysis by PCR. Pathotype ENES/ETES is determined by the presence of diarrheagenic: iha, stx1/2, estII - genes of adhesin, shiga-like toxin and thermostable enterotoxin b, respectively. The APEC pathotype is determined by the presence of genes of extraintestinal groups of Escherichia: hlyF, colV: avian hemolysin, ColV plasmid marker, respectively. The UPEC pathotype is determined by the presence of genes: usp, papGII/III: uropathogenic specific protein, pyelonephritis and cystitis-associated variants of pili, respectively. Epidemic and epizootic significance is determined by the presence of iss, blaCTX-M: survival factor genes in serum and beta-lactamase, respectively. Next, the genetic profile of the culture is compiled and, based on the presence of the corresponding genes, its group is determined: DEC or ExPEC, and pathotype: EHEC/ETEC, APEC or UPEC. The conclusion is also made about the epidemic and epizootic significance of the isolated strain. The method makes it possible to carry out an optimal and adequate assessment of the pathotype (pathogenic potential), as well as the epidemic and epizootic significance of E. coli strains isolated from farm animals and/or circulating in agricultural enterprises.
EFFECT: method provides effective control over the spread of pathogenic and cephalosporin-resistant field E. coli.
1 cl, 2 tbl, 4 ex
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