NEISSERIA MENINGITIDIS COMPOSITIONS AND METHODS Russian patent published in 2022 - IPC C07K14/22 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and is an immunogenic composition capable of causing an immune response against a strain of Neisseria meningitidis serological group A, Neisseria meningitidis serological group C, Neisseria meningitidis serological group W and Neisseria meningitidis serological group Y in humans, containing: a) a liquid composition containing: (i) the first lipidized polypeptide comprising the amino acid sequence presented in SEQ ID NO: 1; and (ii) a second lipidized polypeptide comprising the amino acid sequence presented in SEQ ID NO: 2 and aluminum; and (b) a lyophilized composition containing: i) Neisseria meningitidis serological group A (MenA) capsular saccharide conjugated with adipic acid dihydroside linker (ADH) by 1-cyano-4 tetrafluoroborate-dimethylaminopyridinium, where the specified linker is conjugated with tetanus toxoid (TT) by a carbodiimide chemical method (MenAAH-TT conjugate); ii)capsular saccharide of Neisseria meningitidis of serological group C (MEPs) conjugated with ADH-linker by 1-cyano-4-dimethylaminopyridinium tetrafluoroborate, where said linker is conjugated with tetanus toxoid (TT) by carbodiimide chemical method (MenCAH-TT conjugate); iii) capsular saccharide of Neisseria meningitidis of serological groups W135 (MenW), directly conjugated with tetanus toxoid (TT) by 1-cyano-4-dimethylaminopyridinium tetrafluoroborate in the absence of a linker (MenW-TT conjugate); and iv) capsular saccharide of Neisseria meningitidis of serological group Y (MenY), directly conjugated with tetanus toxoid (TT) by means of 1-cyano-4-dimethylaminopyridinium tetrafluoroborate in the absence of a linker (MenY-TT conjugate), where the lyophilized composition is diluted with a liquid composition.

EFFECT: invention makes it possible to effectively induce an immune response against the strain of Neisseria meningitidis serological group A, Neisseria meningitidis serological group C, Neisseria meningitidis serological group W and Neisseria meningitidis serological group Y in humans.

15 cl, 16 dwg, 31 tbl, 22 ex