METHOD FOR PREPARING TOOTH PREPARATIONS FOR MORPHOLOGICAL STUDIES OF ENAMEL IN AN ATOMIC FORCE MICROSCOPE Russian patent published in 2022 - IPC A61C3/02 A61C3/06 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to dentistry and histology, and can be used to prepare dental preparations for morphological studies of the enamel ultrastructure in an atomic force microscope. Step-by-step and sequential grinding is carried out on the MP-1BGrinderPolisher machine with Dexter grinding wheels with a grain size of 800, 1000, 1500, 2000, 2500 grit with cooling in distilled water and control of the removed surface layer of the drug using a caliper. For this: 1 mm of the outer layer is ground off with a Dexter grinding wheel, 800 grit, cooled with distilled water. The preparation is treated with a Dexter grinding wheel with a grain size of 1000 grit, cooled with distilled water, and ground off 0.5 mm. The preparation is treated with Dexter grinding wheels with a grain size of 1500, 2000, 2500 grit, cooled with distilled water, and ground off 0.5 mm. Next, the preparation is cleaned in an ultrasonic bath for 1 minute, polished on an MP-1BGrinderPolisher grinding and polishing machine using a felt polishing wheel with GOI paste for plastics for 2 minutes, cooled and washed in distilled water. The final polishing is carried out on the MP-1BGrinderPolisher grinding and polishing machine using a plush polishing wheel with Akasel diamond suspension 1 micron for 30 seconds, then surface treatment is carried out in an ultrasonic bath for 2 minutes. After that, the preparation is dried using a building hair dryer at a temperature of 36°C for 30 seconds, the surface is etched with 37% orthophosphoric acid H3PO4 for 20 seconds, and washed under running distilled water for 15 seconds, and dried using a building hair dryer at a temperature of 36°C for 30 seconds.

EFFECT: method makes it possible to study not only enamel prisms, but also individual intra-prismatic and inter-prismatic crystals, inter-prismatic gaps, connecting complexes over the entire polished enamel surface of various layers on a longitudinal section by preparing the sample surface using the claimed methods of the method.

1 cl, 2 dwg, 2 ex