METHOD FOR DETERMINING BLOOD PLASMA COAGULATION INDICATORS - THROMBIN ACTIVITY, PLASMA RECALCIFICATION TIME AND PLASMA TOLERANCE TO HEPARIN Russian patent published in 2022 - IPC G01N33/86 G01N33/52 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to laboratory methods for studying hemostasis, and concerns the determination of blood plasma coagulation parameters - thrombin activity, plasma recalcification time and plasma tolerance to heparin. The fluorescence method is used in the visible range of 420-600 nm in the presence of compounds of the borondipyrine series (BODIPY), such as 4,4-difluoro-8-phenyl-1,3,5,7-tetramethyl-2,6-diethyl-4- boron-3a,4a-diaza-s-indacene; 4,4-difluoro-8-(para-dimethylaminophenyl)-1,3,5,7-tetramethyl-2,6-diethyl-4-boron-3a,4a-diaza-8-indacene; 4,4-difluoro-8-(di-orthomethylphenyl)-1,3,5,7-tetramethyl-2,6-diethyl-4-boron-3a,4a-diaza-s-indacene, whose fluorescence depends on the presence of biomolecules fibrinogen. The dependence of the fluorescence intensity at the maximum emission of BODIPY is recorded, then a coagulation curve of the plasma sample is obtained. In this case, the study is stopped when a stable plateau is reached on the fluorescence change curve. Next, the coagulation curve is analyzed, while three sections are observed and analyzed on the coagulation curve: an upper plateau, a descending section and a lower plateau. At the same time, based on the length of the upper plateau (tlag), the plasma tolerance to heparin is determined. Then a tangent is drawn to the descending section of the coagulation curve, which forms the angle of inclination φ to the y-axis: tg φ, which is proportional to thrombin activity. The plasma recalcification time is obtained by extrapolating the tangent to the descending portion of the coagulation curve to the x-axis (time), cutting off the fibrin clot stabilization step.

EFFECT: invention provides for the expansion of technical capabilities by determining the three indicated indicators of blood plasma coagulation and increasing the sensitivity and accuracy of the process of their determination.

1 cl, 1 dwg, 4 tbl