METHOD FOR DETECTION OF ATYPICAL BRASSICA OLERACEA PLANT Russian patent published in 2023 - IPC C12Q1/686 C12N15/09 C12Q1/6876 

Abstract RU 2787519 C2

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology, in particular it proposes a method for detection of an aneuploid Brassica oleracea plant, including: PCR in real time, using, as a matrix, DNA isolated from a sample obtained from Brassica oleracea plant for testing and DNA markers specific for each of two or more chromosomes of Brassica oleracea plant; and isolation of chromosomic aneuploidy by a relative difference in amplification amount between obtained DNA markers.

EFFECT: according to one of options of implementation of the present invention, it is possible to simply, accurately, and quickly detect an atypical plant (chromosomic aneuploid), which can be found in Brassica oleracea varieties, in a laboratory equipped with general molecular-biological equipment during quality control of seeds and breeding researches.

13 cl, 7 dwg, 7 tbl, 5 ex

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RU 2 787 519 C2

Authors

Izumida, Atsushi

Suzuki, Takao

Hiramoto, Tetsuya

Dates

2023-01-10Published

2018-08-17Filed