FIELD: cell biology.
SUBSTANCE: invention relates to cell biology, in particular to a membrane for separating target stem cells from biological samples. As a result, sterile target stem cells are obtained in a physiological buffer. The specified membrane consists of a three-dimensional carrier structure made of at least one layer of a biocompatible polymer with a certain pore size, as a carrier material, and covalently bound target molecules on its surface and/or in the pores. These target molecules are preferably target antibodies that recognize characteristic antigens that are bound on the surface of the target stem cells and thus bind the target stem cells to the membrane. The target molecules can either be directly bound to the surface and/or in the pores of the carrier structure, or connected to the surface and/or in the pores of the carrier structure through specific functionalized nanoparticles that are bound or embedded in the three-dimensional carrier structure of the membrane. In addition, the present invention includes a method for obtaining a membrane, as well as a method and device for separating target stem cells from a biological sample, which include the above-mentioned membrane as an integral part.
EFFECT: present invention makes it possible to selectively separate selected target stem cells, i.e. to bind only certain target stem cells from a cell suspension that contains many different cells, passing all other unwanted cells through the membrane.
43 cl, 1 dwg, 2 ex
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