METHOD FOR DETERMINING THE RATE OF ACCUMULATION OF CADMIUM IN THE BRAIN OF RATS Russian patent published in 2023 - IPC G01N33/50 C12Q1/6806 C12Q1/686 C12Q1/6876 C12Q1/6883 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to toxicology, and can be used to determine the rate of accumulation of cadmium in the brain of rats. RNA is isolated from the peripheral venous blood lymphocytes. The amount of mRNA of the ZIP1 gene is determined. The amount of mRNA is normalized to endogenous control of GAPDH. When the multiplicity of the expression of the ZIP1 gene relative to the expression of GAPDH is from -10 to -0.1, a low rate of accumulation of cadmium in the brain of rats is determined, which is 0.02±0.002 mg/kg/month, from 0.1 to 5 - average accumulation rate of 0.04±0.004 mg/kg/month, from 5.1 to 10 - a high rate of accumulation of cadmium in the kidneys, which is 0.08± 0.007 mg/kg/month.

EFFECT: the method improves the accuracy of determination of cadmium in the brain by estimating the rate of accumulation of cadmium in the blood.

1 cl, 9 ex