METHOD FOR DETERMINING THE RATE OF ACCUMULATION OF CADMIUM IN THE LIVER OF RATS Russian patent published in 2023 - IPC G01N33/50 C12Q1/6806 C12Q1/686 C12Q1/6876 C12Q1/6883 

FIELD: medicine.

SUBSTANCE: in toxicology it can be used to determine the rate of accumulation of cadmium in the liver of rats. RNA is isolated from the peripheral venous blood lymphocytes, the amount of mRNA of the MT3A gene is determined. Then amount of mRNA is normalized to endogenous control of GAPDH. When the multiplicity of the expression of the MT3A gene relative to the expression of GAPDH is from -10 to -0.1, a low rate of accumulation of cadmium in the liver of rats is determined, which is 0.3± 0.03 mg/kg/month. When the multiplicity of the expression of the MT3A gene relative to the expression of GAPDH is from 0.1 to 5, a moderate rate of accumulation is determined, which is 0.6±0.04 mg/kg/month When the multiplicity of the expression of the MT3A gene relative to the expression of GAPDH is from 5.1 to 10, a high rate of accumulation of cadmium in the liver is determined, which is 1.2±0.06 mg/kg/month.

EFFECT: increasing the accuracy of determining the content of cadmium in the liver by estimating the rate of its accumulation.

1 cl, 9 ex